Detection and Application of Molecular Markers for Functional Genes of Important Traits in Tomato
SHI Qianqian1，2，LU Xiaonan2，LI Ren2，LI Sen1，SUN Shuai2*，CUI Xia2*
1College of Horticulture，Shanxi Agricultural University，Taigu 030801，Shanxi，China；2State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
Abstract：To achieve high-throughput genotyping and molecular marker-assisted selection（MAS）in
tomato，22 molecular markers related to tomato disease resistance（Ty-1，Ty-2，Ty-3a，Ty-5，Sm，Rx4，
Tm-2，Pto，I-1，Frl，Mi-1，Bwr-12，Ph-3），sugar and acid content（STP，Lin5，ALMT9），fruit
hardness（FIS1），fruit shape（OVATE），inflorescence branch（J2，EJ2，STM3）and plant type（SP）
were developed. The results of genotyping showed that 22 markers could accurately classify heterozygous and two homozygous genotypes. For disease resistance，the genotypes of 27 tomato varieties showed that 90% of the tested varieties contained fi ve or more disease resistance genes，and 16 varieties contained 7-9 disease resistance genes. Six tomato varieties contained high sugar，high acid and multi-infl orescence branching genes.In addition，effi cient KASP markers were developed and genotyped for eight of these key genes to verify the stability of the molecular markers. In conclusion，the molecular markers developed in this study have important application value for improving the efficiency of resistance breeding and quality breeding in tomato and for accelerating the breeding of new varieties with high yield，high quality and disease resistance
史茜茜，逯晓楠，李仁，李森，孙帅，崔霞. 番茄重要性状功能基因的分子标记检测及应用[J]. 中国蔬菜, 2024, 1(11): 27-38.
SHI Qianqian，LU Xiaonan，LI Ren，LI Sen，SUN Shuai，CUI Xia. Detection and Application of Molecular Markers for Functional Genes of Important Traits in Tomato. China Vegetables, 2024, 1(11): 27-38.
